A physical map of human chromosome 14

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Brüls, Thomas | Gyapay, Gabor | Petit, Jean-Louis | Artiguenave, François | Vico, Virginie | Qin, Shizen | Tin-Wollam, Aye Mon | da Silva, Corinne | Muselet, Delphine | Mavel, Delphine | Pelletier, Eric | Levy, Michael | Fujiyama, Asao | Matsuda, Fumihiko | Wilson, Richard | Rowen, Lee | Hood, Leroy | Weissenbach, Jean | Saurin, William | Heilig, Roland

Edité par CCSD ; Nature Publishing Group -

International audience. We report the construction of a tiling path of around 650 clones covering more than 99% of human chromosome 14. Clone overlap information to assemble the map was derived by comparing fully sequenced clones with a database of clone end sequences (sequence tag connector strategy). We selected homogeneously distributed seed points using an auxiliary high-resolution radiation hybrid map comprising 1,895 distinct positions. The high long-range continuity and low redundancy of the tiling path indicates that the sequence tag connector approach compares favourably with alternative mapping strategies

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