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Tailoring confocal microscopy for real-time analysis of photosynthesis at single-cell resolution
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Edité par CCSD ; Cell Press Elsevier -
International audience. Photoautotrophs’ environmental responses have been extensively studied at the organism and ecosystemlevel. However, less is known about their photosynthesis at the single-cell level. This information is neededto understand photosynthetic acclimation processes, as light changes as it penetrates cells, layers of cells, ororgans. Furthermore, cells within the same tissue may behave differently, being at different developmental/physiological stages. Here, we describe an approach for single-cell and subcellular photophysiology basedon the customization of confocal microscopy to assess chlorophyll fluorescence quenching by the saturationpulse method. We exploit this setup to (1) reassess the specialization of photosynthetic activities in developingtissues of non-vascular plants; (2) identify a specific subpopulation of phytoplankton cells in marinephotosymbiosis, which consolidate energetic connections with their hosts; and (3) examine the link betweenlight penetration and photoprotection responses inside the different tissues that constitute a plant leafanatomy.
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