FUNCTIONAL CHARACTERIZATION OF THE XANTHOMONASCAMPESTRIS TALOME

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Audran, Corinne | Brice, Charleux | Zárate-Chaves, Carlos Andrés | Denancé, Nicolas | Fuentes, Ivanna | Bellenot, Caroline | Gris, Carine | Carrere, Sébastien | Jardinaud, Marie‐françoise | Bernal, Adriana | Szurek, Boris | Noël, Laurent D.

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International audience. Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot disease,which is the most important and destructive bacterial disease of Brassica crops. MostXanthomonas species translocate Transcription Activator-Like Effectors (TALEs) proteinsinside plant cells using their type III secretion machinery. TALEs are a unique type ofbacterial effector proteins that help the pathogen to exploit and modify the plant environmentby directly altering the expression of plant genes to the benefit of the bacteria. TALEs play anessential role in many plant diseases caused by Xanthomonas spp. but the contribution ofTALEs to virulence of Xcc has not been characterized. We identified a rich repertoire of talgenes (TALome) in Xcc and studied its functional role in disease development. First, wesuccessfully silenced the tal genes in two Xcc strains using CRISPRi technology,demonstrating the role of TALEs for Xcc pathogenicity. In addition, the contribution ofindividual Xcc TALEs to bacteria virulence was tested by gain and loss-of-functionexperiments. Our data provide a platform to explore the roles of TALEs in black rot disease.We will present our latest results on the mechanisms of TALE-mediated susceptibility inBrassicaceae

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