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PNPase is involved in the coordination of mRNA degradation and expression in stationary phase cells of Escherichia coli

Archive ouverte | Dressaire, Clémentine | CCSD

Background: Exoribonucleases are crucial for RNA degradation in Escherichia coli but the roles of RNase R and PNPase and their potential overlap in stationary phase are not well characterized. Here, we used a genome-wide approach ...

When translation elongation is impaired, the mRNA is uniformly destabilized by the RNA degradosome, while the concentration of mRNA is altered along the molecule

Archive ouverte | Duviau, Marie-Pierre | CCSD

International audience. mRNA sits at the crossroads of transcription, translation and mRNA degradation. Many questions remain about the coupling of these three processes in Escherichia coli and, in particular, how t...

Multiplexing polysome profiling experiments to study translation in Escherichia coli

Archive ouverte | Nguyen, Huong Le | CCSD

Polysome profiling is a widely used method to monitor the translation status of mRNAs. Although it is theoretically a simple technique, it is labor intensive. Repetitive polysome fractionation rapidly generates a large number of s...

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