0 avis
TRAP2 model for studying activated structures during Paradoxical (REM) Sleep
Archive ouverte
Edité par CCSD -
International audience. Our aim is to identify the populations of neurons activated during paradoxical (REM) sleep and to determine whether introduction of adverse or learning events induce the activation of different populations of neurons than those seen in basal conditions. To this aim, we are using a new genetic TRAP method (2) combining tdtomato expression under Fos promoter with Fos immunohistochemistry. We first examined 3 mice groups: PSR-PSR (2h PS recovery after 48h PS deprivation), Wake-Wake (2h in open field), and Wake-PSR group all receiving 4-OHT 2h after the first condition and perfused 2h after the second condition. We then performed immunofluorescence of cFos and automatic counting of neurons on brain sections of the mice taken every 240um. Overall, more cFos and tdtomato labeled neurons were observed in most structures during the W compared to the PSR condition. The dentate gyrus was one of the few structures containing more cFos neurons after PSR than W. As reported recently for the anterior cingulate, retrosplenial and claustrum with DAB staining (Maciel et al., 2021), less double-labeled neurons were observed in the W-PSR than in the W-W and PSR-PSR conditions in most brain structures examined. The development of a new method combining the use of TRAP mice with automatic counting open the path to the study over the whole brain of populations of neurons activated during two different conditions. Further, our results indicate that W and PS are completely different states during which different populations of neurons are activated.
Consulter en ligne
Suggestions
Du même auteur
