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Calcifications in human osteoarthritic articular cartilage: ex vivo assessment of calcium compounds using XANES spectroscopy

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Nguyen, Christelle | Ea, Hang Korng | Thiaudiere, Dominique | Reguer, Solenn | Hannouche, Didier | Daudon, Michel | Lioté, Frédéric | Bazin, Dominique

Edité par CCSD ; International Union of Crystallography -

International audience. Calcium (Ca2+)-containing crystals (CCs), including basic Ca2+ phosphate(BCP) and Ca2+ pyrophosphate dihydrate (CPPD) crystals, are associated withsevere forms of osteoarthritis (OA). Growing evidence supports a role forabnormal articular cartilage mineralization in the pathogenesis of OA.However, the role of Ca2+ compounds in this mineralization process remainspoorly understood. Six patients, who underwent total knee joint replacement forprimary OA, have been considered in this study. Cartilage from femoralcondyles and tibial plateaus in the medial and lateral compartments wascollected as 1 mm-thick slices cut tangentially to the articular surface. First, CCspresence and biochemical composition were assessed using Fourier transforminfrared spectroscopy (FT-IR). Next, Ca2+ compound biochemical form wasfurther assessed using X-ray absorption spectroscopy (XAS) performed at theCa2+ K-absorption edge. Overall, 12 cartilage samples were assessed. Using FTIR,BCP and CPPD crystals were detected in four and three out of 12 samples,respectively. Ca2+ compound biochemical forms differed between areas withversus without CCs, when compared using XAS. The complete set of data showsthat XANES spectroscopy can be used to accurately characterize sparse CCs inhuman OA cartilage. It is found that Ca2+ compounds differ between calcifiedand non-calcified cartilage areas. In calcified areas they appear to be mainlyinvolved in calcifications, namely Ca2+ crystals.

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