Application of small angle scattering (SAS) in structural characterisation of casein and casein-based products during digestion

Archive ouverte

Bayrak, Meltem | Mata, Jitendra | Conn, Charlotte | Floury, Juliane | Logan, Amy

Edité par CCSD ; Elsevier -

International audience. In recent years, small and ultra-small angle scattering techniques, collectively known as small angle scattering (SAS) have been used to study various food structures during the digestion process. These techniques play an important role in structural characterisation due to the non-destructive nature (especially when using neutrons), various in situ capabilities and a large length scale (of 1 nm to ∼20 μm) they cover. The application of these techniques in the structural characterisation of dairy products has expanded significantly in recent years. Casein, a major dairy protein, forms the basis of a wide range of gel structures at different length scales. These gel structures have been extensively researched utilising scattering techniques to obtain structural information at the nano and micron scale that complements electron and confocal microscopy. Especially, neutrons have provided opportunity to study these gels in their natural environment by using various in situ options. One such example is understanding changes in casein gel structures during digestion in the gastrointestinal tract, which is essential for designing personalised food structures for a wide range of food-related diseases and improve health outcomes. In this review, we present an overview of casein gels investigated using small angle and ultra-small angle scattering techniques. We also reviewed their digestion using newly built setups recently employed in various research. To gain a greater understanding of micro and nano-scale structural changes during digestion, such as the effect of digestive juices and mechanical breakdown on structure, new setups for semi-solid food materials are needed to be optimised.

Consulter en ligne

Suggestions

Du même auteur

Correlating structure and activity of pepsin enzyme in H2O and D2O for the study of gastric digestion

Archive ouverte | Bayrak, Meltem | CCSD

International audience. D2O, an isotope of H2O, is commonly used as a solvent in neutron scattering; the large difference in scattering length density between H and D can provide better contrast between the sample a...

Effect of the differences in protein structure on digestibility

Archive ouverte | Bayrak, Meltem | CCSD

Food is a complex combination of chemically diverse structures, influenced by composition andprocessing. The nutritional quality of food cannot be solely based on individual contents in nutrientsas food structure will strongly mod...

Investigating casein gel structure during gastric digestion using ultra-small and small-angle neutron scattering

Archive ouverte | Bayrak, Meltem | CCSD

International audience. This study aimed to understand the structural devolution of 10% w/w rennet-induced (RG) and transglutaminase-induced acid (TG) gels in H2O and D2O under in vitro gastric conditions with and w...

Chargement des enrichissements...