Afficher la couverture 'Barcoding T Cell Calcium Response Diversity with Methods for Automated and Accurate Analysis of Cell Signals (MAAACS) | Salles, Audrey' en grand format
/5

0 avis

Barcoding T Cell Calcium Response Diversity with Methods for Automated and Accurate Analysis of Cell Signals (MAAACS)

Archive ouverte

Salles, Audrey | Billaudeau, Cyrille | Sergé, Arnauld | Bernard, Anne-Marie | Phélipot, Marie-Claire | Bertaux, Nicolas | Fallet, Mathieu | Grenot, Pierre | Marguet, Didier | He, Hai-Tao | Hamon, Yannick

Edité par CCSD ; PLOS -

International audience. We introduce a series of experimental procedures enabling sensitive calcium monitoring in T cell populations by confocal video-microscopy. Tracking and post-acquisition analysis was performed using Methods for Automated and Accurate Analysis of Cell Signals (MAAACS), a fully customized program that associates a high throughput tracking algorithm, an intuitive reconnection routine and a statistical platform to provide, at a glance, the calcium barcode of a population of individual T-cells. Combined with a sensitive calcium probe, this method allowed us to unravel the heterogeneity in shape and intensity of the calcium response in T cell populations and especially in naive T cells, which display intracellular calcium oscillations upon stimulation by antigen presenting cells.

Suggestions

Du même auteur

Glycosylation-Dependent IFN-gamma R Partitioning in Lipid and Actin Nanodomains Is Critical for JAK Activation

Archive ouverte | Blouin, Cedric M. | CCSD

International audience. Understanding how membrane nanoscale organization controls transmembrane receptors signaling activity remains a challenge. We studied interferon-gamma receptor (IFN-gamma R) signaling in fibr...

Raft nanodomains contribute to Akt/PKB plasma membrane recruitment and activation.

Archive ouverte | Lasserre, Rémi | CCSD

International audience. Membrane rafts are thought to be sphingolipid- and cholesterol-dependent lateral assemblies involved in diverse cellular functions. Their biological roles and even their existence, however, r...

Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes.

Archive ouverte | Sergé, Arnauld | CCSD

International audience. Although the highly dynamic and mosaic organization of the plasma membrane is well-recognized, depicting a resolved, global view of this organization remains challenging. We present an analyt...

Chargement des enrichissements...