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Virulent coliphages in 1-year-old children fecal samples are fewer, but more infectious than temperate coliphages
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Edité par CCSD ; Nature Publishing Group -
International audience. Bacteriophages constitute an important part of the human gut microbiota, but their impact on this community is largely unknown. We cultivated temperate phages produced by 900 E. coli strains isolated from 648 fecal samples of children 1-year-old. Next, we isolated coliphages directly from the viral fraction of the same fecal samples. We found that 63% of strains hosted phages, while 24% of the viromes contained phages targeting E. coli. 150 of these phages, half recovered from strain supernatants, half from virome (73% temperate and 27% virulent) were tested for their host range on 75 E. coli strains isolated from the same cohort. Temperate phages barely infected the gut strains, whereas virulent phages killed up to 68% of them. We conclude that in fecal samples from children, temperate coliphages dominate, while virulent ones have greater infectivity and broader host range, likely playing a role in gut microbiota dynamics.
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