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Screening of specific aptamers for Bacillus cereus by Surface Plasmon Resonance imaging (SPRi)

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Manceau, Mathilde | Farre, Carole | Lagarde, Florence | Mathey, Raphaël | Buhot, Arnaud | Vidic, Jasmina | Hou, Yanxia | Chaix, Carole

Edité par CCSD -

International audience. Bacillus cereus is a food poisoning bacterium present in dairy products. To detect it, antibody based biosensors are efficient but available antibodies are expensive and may cross-react with other Bacillus species. Aptamers are a good alternative to these issues. Herein, seven aptamer sequences derived from the literature (1,2) are compared and their specificity to B. cereus is assessed versus Escherichia coli and Bacillus subtilis by Surface Plasmon Resonance imaging (SPRi).

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