Syracuse

DRAGin situbarcoding reveals an increased number of HSPCs contributing to myelopoiesis with age

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Urbanus, Jos | Cosgrove, Jason | Beltman, Joost | Elhanati, Yuval | de Andrade Moral, Rafael | Conrad, Cecile | van Heijst, Jeroen, W | Tubeuf, Emilie | Velds, Arno | Kok, Lianne | Merle, Candice | Magnusson, Jens, P | Frisén, Jonas | Fre, Silvia | Walczak, Aleksandra, M | Mora, Thierry | Jacobs, Heinz | Schumacher, Ton, N | Perié, Leïla

Edité par CCSD -

Ageing is associated with changes in the cellular composition of the immune system. During ageing, hematopoietic stem and progenitor cells (HSPCs) that produce immune cells are thought to decline in their regenerative capacity. However, HSPC function has been mostly assessed using transplantation assays, and it remains unclear how HSPCs age in the native bone marrow niche. To address this issue, we developed a novel in situ single cell lineage tracing technology to quantify the clonal composition and cell production of single cells in their native niche. Our results demonstrate that a pool of HSPCs with unequal output maintains myelopoiesis through overlapping waves of cell production throughout adult life. During ageing, the increased frequency of myeloid cells is explained by greater numbers of HSPCs contributing to myelopoiesis, rather than increased myeloid output of individual HSPCs. Strikingly, the myeloid output of HSPCs remained constant over time despite accumulating significant transcriptomic changes throughout adulthood. Together, these results show that, unlike emergency myelopoiesis posttransplantation, aged HSPCs in their native microenvironment do not functionally decline in their regenerative capacity.

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