Fluorogenic and genetic targeting of a red-emitting molecular calcium indicator

Archive ouverte

Bachollet, Sylvestre | Pietrancosta, Nicolas | Mallet, Jean-Maurice | Dumat, Blaise

Edité par CCSD ; Royal Society of Chemistry -

International audience. The fluorogenic activation of a calcium molecular probe via reaction with HaloTag yields a fluorescent AND logic gate enabling selective and wash-free calcium imaging in genetically-defined organelles.

Suggestions

Du même auteur

An expanded palette of fluorogenic HaloTag probes with enhanced contrast for targeted cellular imaging

Archive ouverte | Bachollet, Sylvestre | CCSD

International audience. We report the development of HaloTag fluorogens based on dipolar flexible molecular rotor structures. By modulating the electron donating and withdrawing groups, we have tuned the absorption ...

Fluorogenic Protein Probes with Red and Near‐Infrared Emission for Genetically Targeted Imaging

Archive ouverte | Bachollet, Sylvestre | CCSD

International audience. Fluorogenic probes are important tools to image proteins with high contrast and no wash protocols. In this work, we rationally designed and synthesized a small set of four protein fluorogens ...

FRET-sensing of receptor binding at the interface of targeted biomimetic microparticles functionalized with tunable fluorescent lipids

Archive ouverte | Michelis, Sophie | CCSD

Cell adhesion is a fundamental phenomenon for cell communication and regulation. Adhesion sites are triggered by the binding of single ligand-receptor pairs that will initiate the formation of clusters of receptors. To study cell ...

Chargement des enrichissements...