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Whole Animal Multiplexed Single-Cell RNA-Seq Reveals Plasticity of Clytia Medusa Cell Types
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Abstract We present an organism-wide, transcriptomic cell atlas of the hydrozoan medusa Clytia hemisphaerica , and determine how its component cell types respond to starvation. Utilizing multiplexed scRNA-seq, in which individual animals were indexed and pooled from control and perturbation conditions into a single sequencing run, we avoid artifacts from batch effects and are able to discern shifts in cell state in response to organismal perturbations. This work serves as a foundation for future studies of development, function, and plasticity in a genetically tractable jellyfish species. Moreover, we introduce a powerful workflow for high-resolution, wh ole a nimal, m ultiplexed single-cell genomics (WHAM-seq) that is readily adaptable to other traditional or non-traditional model organisms.
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