ABCG transporters export cutin precursors for the formation of the plant cuticle

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Elejalde-Palmett, Carolina | Martinez San Segundo, Ignacio | Garroum, Imène | Charrier, Laurence | de Bellis, Damien | Mucciolo, Antonio | Guerault, Aurore | Liu, Jie | Zeisler-Diehl, Viktoria | Aharoni, Asaph | Schreiber, Lukas | Bakan, Benedicte | Clausen, Mads | Geisler, Markus | Nawrath, Christiane

Edité par CCSD ; Elsevier -

International audience. The plant cuticle is deposited on the surface of primary plant organs, such as leaves, fruits, and floral organs,forming a diffusion barrier and protecting the plant against various abiotic and biotic stresses. Cutin, thestructural polyester of the plant cuticle, is synthesized in the apoplast. Plasma-membrane-localized ATP-binding cassette (ABC) transporters of the G family have been hypothesized to export cutin precursors.Here, we characterizeSlABCG42of tomato representing an ortholog ofAtABCG32inArabidopsis.SlABCG42expression inArabidopsiscomplements the cuticular deficiencies of theArabidopsis pec1/abcg32mutant.RNAi-dependent downregulation of both tomato genes encoding proteins highly homologous to AtABCG32(SlABCG36 and SlABCG42) leads to reduced cutin deposition and formation of a thinner cuticle in tomatofruits. By using a tobacco (Nicotiana benthamiana) protoplast system, we show that AtABCG32 andSlABCG42 have an export activity for 10,16-dihydroxy hexadecanoyl-2-glycerol, a cutin precursorin vivo.Interestingly, also freeu-hydroxy hexadecanoic acid as well as hexadecanedioic acid were exported,furthering the research on the identification of cutin precursorsin vivoand the respective mechanisms of their integration into the cutin polymer.

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