Afficher la couverture 'Organoid Sample Preparation and Extraction for LC-MS Peptidomics | Miedzybrodzka, Emily, L' en grand format
/5

0 avis

Organoid Sample Preparation and Extraction for LC-MS Peptidomics

Archive ouverte

Miedzybrodzka, Emily, L | Foreman, Rachel, E | Galvin, Sam, G | Larraufie, Pierre | George, Amy, L | Goldspink, Deborah, A | Reimann, Frank | Gribble, Fiona, M | Kay, Richard, G

Edité par CCSD ; Elsevier -

International audience. This protocol describes the peptidomic analysis of organoid lysates, FACS-purified cell populations, and 2D culture secretions by liquid chromatography mass spectrometry (LC-MS). Currently, most peptides are quantified by ELISA, limiting the peptides that can be studied. However, an LC-MS-based approach allows more peptides to be monitored. Our group has previously used LC-MS for tissue peptidomics and secretion of enteroendocrine peptides from primary culture. Now, we extend the use to organoid models.

Suggestions

Du même auteur

Stimulation of motilin secretion by bile, free fatty acids, and acidification in human duodenal organoids

Archive ouverte | Miedzybrodzka, Emily, L | CCSD

International audience. Objective: Motilin is a proximal small intestinal hormone with roles in gastrointestinal motility, gallbladder emptying, and hunger initiation. In vivo motilin release is stimulated by fats, ...

Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling

Archive ouverte | Roberts, Geoffrey, P | CCSD

International audience

Single cell transcriptomic profiling of large intestinal enteroendocrine cells in mice – Identification of selective stimuli for insulin-like peptide-5 and glucagon-like peptide-1 co-expressing cells

Archive ouverte | Billing, Lawrence, J | CCSD

International audience. Objective: Enteroendocrine cells (EECs) of the large intestine, found scattered in the epithelial layer, are known to express different hormones, with at least partial co-expression of differ...

Chargement des enrichissements...