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Millisecond time-resolved serial oscillation crystallography of a blue-light photoreceptor at a synchrotron

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Aumonier, Sylvain | Santoni, Gianluca | Gotthard, Guillaume | von Stetten, David | Leonard, Gordon A. | Royant, Antoine

Edité par CCSD ; International Union of Crystallography -

International audience. The recent development of serial crystallography has popularized time-resolvedcrystallography as a technique to determine the structure of protein-reactionintermediate states. However, most approaches rely on the availability ofthousands to millions of microcrystals. A method is reported here, usingmonochromatic synchrotron radiation, for the room-temperature collection,processing and merging of X-ray oscillation diffraction data from <100 samplesin order to observe the build up of a photoreaction intermediate species. Usingthis method, we monitored with a time resolution of 63 ms how the populationof a blue-light photoreceptor domain in a crystal progressively photoconvertsfrom the dark to the light state. The series of resulting snapshots allows us tovisualize in detail the gradual rearrangement of both the protein andchromophore during this process.

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