Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning

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Mazaré, Noémie | Oudart, Marc | Moulard, Julien | Cheung, Giselle | Tortuyaux, Romain | Mailly, Philippe | Mazaud, David | Bemelmans, Alexis-Pierre | Boulay, Anne-Cécile | Blugeon, Corinne | Jourdren, Laurent | Le Crom, Stéphane | Rouach, Nathalie | Cohen-Salmon, Martine

Edité par CCSD ; Elsevier Inc -

International audience. Local translation is a conserved mechanism conferring cells the ability to quickly respond to local stimuli. Inthe brain, it has been recently reported in astrocytes, whose fine processes contact blood vessels and synapses.Yet the specificity and regulation of astrocyte local translation remain unknown. We study hippocampalperisynaptic astrocytic processes (PAPs) and show that they contain the machinery for translation. Usinga refined immunoprecipitation technique, we characterize the entire pool of ribosome-bound mRNAs in PAPsand compare it with the one expressed in the whole astrocyte. We find that a specific pool of mRNAs is highlypolarized at the synaptic interface. These transcripts encode an unexpected molecular repertoire, composedof proteins involved in iron homeostasis, translation, cell cycle, and cytoskeleton. Remarkably, we observealterations in global RNA distribution and ribosome-bound status of some PAP-enriched transcripts afterfear conditioning, indicating the role of astrocytic local translation in memory and learning.

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