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A Role for Taok2 in Listeria monocytogenes Vacuolar Escape

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Quereda, Juan | Morel, Camille | Lopez-Montero, Noelia | Ziveri, Jason | Rolland, Steven | Grenier, Théodore | Aulner, Nathalie | Danckaert, Anne | Charbit, Alain | Enninga, Jost | Cossart, Pascale | Pizarro-Cerdá, Javier

Edité par CCSD ; Oxford University Press -

International audience. The bacterial pathogen Listeria monocytogenes invades host cells, ruptures the internalization vacuole, and reaches the cytosol for replication. A high-content small interfering RNA (siRNA) microscopy screen allowed us to identify epithelial cell factors involved in L. monocytogenes vacuolar rupture, including the serine/threonine kinase Taok2. Kinase activity inhibition using a specific drug validated a role for Taok2 in favoring L. monocytogenes cytoplasmic access. Furthermore, we showed that Taok2 recruitment to L. monocytogenes vacuoles requires the presence of pore-forming toxin listeriolysin O. Overall, our study identified the first set of host factors modulating L. monocytogenes vacuolar rupture and cytoplasmic access in epithelial cells.

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