In‐Cell EPR: Progress towards Structural Studies Inside Cells

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Bonucci, Alessio | Ouari, Olivier | Guigliarelli, Bruno | Belle, Valérie | Mileo, Elisabetta

Edité par CCSD ; Wiley-VCH Verlag -

International audience. Exploring biomolecules' structure and dynamics in the context of their intracellular environment has become the ultimate challenge for structural biology. As the cellular environment is barely reproducible in vitro, investigation of biomolecules directly inside cells has attracted a growing interest. Among magnetic resonance approaches, Site-Directed Spin Labeling (SDSL) coupled to Electron Paramagnetic Resonance (EPR) spectroscopy provides competitive and advantageous features to capture protein structure and dynamics inside cells. To date, several in-cell EPR approaches have been successfully applied to both bacterial and eukaryotic cells. In this review, the major advances of in-cell EPR are summarized, as well as the challenges this approach still poses.

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