HepaRG Self-Assembled Spheroids in Alginate Beads Meet the Clinical Needs for Bioartificial Liver

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Pasqua, Mattia | Pereira, Ulysse | Messina, Antonietta | de Lartigue, Claire | Vigneron, Pascale | Dubart-Kupperschmitt, Anne | Legallais, Cécile

Edité par CCSD ; Mary Ann Liebert -

International audience. In liver tissue engineering, cell culture in spheroids is now wellrecognized to promote the maintenance of hepatic functions. However,the process leading to spheroids formation is time consuming, costly,and not easy to scale-up for further use in human bioartificial liver (BAL)applications. In this study, we encapsulated HepaRG cells (precursors ofhepatocyte-like cells) in alginate 1.5% without pre-forming spheroids.Starting from a given hepatic biomass, we analysed cell differentiationand metabolic performance for further use in fluidized-bed BAL. Weobserved that cells rearranged as aggregates into the beads andadequately differentiated over time, in the absence of any differentiatingfactors classically used. At day 14 post-encapsulation, cells displayed a large set of hepatic features necessary for the treatment of a patient inacute liver failure. These activities include albumin synthesis, ammoniaand lactate detoxification and the efficacy of the enzymes involved in thexenobiotic metabolism (such as CYP1A1/2).

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