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Light-powered CO 2 fixation in a chloroplast mimic with natural and synthetic parts

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Miller, Tarryn | Beneyton, Thomas | Schwander, Thomas | Diehl, Christoph | Girault, Mathias | Mclean, Richard | Chotel, Tanguy | Claus, Peter | Cortina, Niña Socorro | Baret, Jean-Christophe | Erb, Tobias

Edité par CCSD ; American Association for the Advancement of Science (AAAS) -

International audience. Nature integrates complex biosyntheticand energy converting tasks within compartments such as chloroplasts or mitochondria. Chloroplasts convert light into chemical energy, driving CO2fixation. We usedmicrofluidics to develop a chloroplast-mimic by encapsulating and operating photosynthetic membranes in cell-sized droplets. Thesedropletscan be energized by light to power enzymes or enzyme cascades and analyzed for their catalytic properties in multiplex and real-time. We demonstrate how the micro-droplets can be programmed and controlled by adjusting internal compositions as well as using light asanexternal trigger. We showcase the capability of our platform by integrating the CETCH cycle, a synthetic network for CO2conversion,tocreate an artificial photosynthetic system that interfaces the natural and the synthetic biological world.

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