Whole-cell voltage-clamp on skeletal muscle fibers with the silicone-clamp technique.

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Pouvreau, Sandrine | Collet, Claude | Allard, Bruno | Jacquemond, Vincent

Edité par CCSD ; Humana Press -

International audience. Control of membrane voltage and membrane current measurements are of strong interest for the study of numerous aspects of skeletal muscle physiology and pathophysiology. The silicone-clamp technique makes use of a conventional patch-clamp apparatus to achieve whole-cell voltage-clamp of a restricted portion of a fully differentiated adult skeletal muscle fiber. The major part of an isolated muscle fiber is insulated from the extracellular medium with silicone grease and the tip of a single microelectrode connected to the amplifier is then inserted within the fiber through the silicone layer. This method represents an alternative to the traditional vaseline-gap isolation- and 2 or 3 microelectrodes voltage-clamp techniques. The present chapter reviews the main benefits of the silicone-clamp technique and provides detailed insights into its practical implementation

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