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Prnp knockdown in transgenic mice using RNA interference

Archive ouverte | Gallozzi, Micaela | CCSD

International audience. RNA interference has become a widely used approach to perform gene knockdown experiments in cell cultures and more recently transgenic animals. A designed miRNA targeting the prion protein mR...

Spatial and temporal down-regulation of transgene expression using the TRSID-silencer in mice: Application to Prnp

Archive ouverte | Gallozzi, Micaela | CCSD

International audience. Spatial and temporal control of ovine prion protein (Prnp) gene expression was achieved in mice using two transgenes: a Prnp minigene with tet-operator sequences inserted 50 to exon 1 and a m...

Mouse Mammary Tumor Virus driven α-lactalbumin expression effects on lactation and fertility of transgenic mice

Archive ouverte | Le Guillou, Sandrine | CCSD

Chantier qualité GA. α-Lactalbumin (Alac) is one of the major milk proteins. Its gene expression is restricted to epithelial cells of the lactating mammary gland. The Alac interaction with a uridine 5′-diphosphate-g...

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