0 avis
Tolerance to high temperature and genetic diversity of black poplar families from two environmental contrasting sites
Archive ouverte
Edité par CCSD -
International audience. The European black poplar is an ecological pioneer tree species, adapted to dynamic riparian zones such as river valley floodplains. Our work was focused on studying the consequences of increasing temperature on genetic diversity of two provenances of Populus nigra L. coming from two contrasting sites: 1) the Loire river with mild climatic conditions and minimum artificialwater level in summer and 2) the Paglia river with higher water-level fluctuations and semi-arid conditions. We collected seeds, as open pollinated families,from 8 mother trees on each of the two different sites. Seeds were grown for 7 weeks in two growth chambers at 25°C. At this time (t0), in one chamber temperature was kept at 25°C and in the second one the temperature was progressively increased to 43°C. The number of individuals with symptoms of wilting were recorded at three successive times (t1, t2 and t3). Nine microsatellite markers were used to measure the genetic diversity between the two provenances, among individuals within the same collection site and among individuals within the 16 open pollinated families. At the increasing temperature, differences in temperature tolerance were observed among families in both provenances. Genetic indices as observed and expected heterozygosity (Ho and He), Shannon genetic diversity index (I) and fixation index (F) were calculated. Differenceson genetic diversity and fixation indexwere detected between provenances as well as between families of the same site.
Consulter en ligne
Suggestions
Du même auteur
