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Effect of a lipid-garlic treatment on archaeal community establishment and rumen function in lambs
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Edité par CCSD ; Academic Publishers -
Session 51 : Poster 26. International audience. The microbial colonization of the rumen after birth is influenced by several factors that may modulate the microbiota composition and function. We report here the effects of a treatment applied in early life on rumen fermentation and archaeal community structure in lambs from birth up to 4 weeks of age. Sixty lambs and their dams were allocated to two groups. Treatment (T) consisted in linseed oil and garlic essential oil drenched daily during the whole experimental period. T was also applied to dams. Rumen contents were sampled by oral stomach tubing. All lambs were sampled at 1 week, as well as all dams for comparison. A representative set of lambs (n=10) was sampled at 2, 3 and 4 weeks. Volatile fatty acids (VFA) were analysed and 16S rDNA amplicon sequencing was used to explore the archaeal diversity. Total VFA concentration was not affected by the treatment except in 1-week-old lambs (34.4 vs 24.3 mM, for C and T, respectively, P<0.05); VFA profile was mostly affected in T dams with lower proportion of acetic (64.5 vs 71.1%, P<0.001) and higher proportion of propionic acid (20.6 vs 13.8%, P<0.001). In lambs, archaeal diversity increased with age but changes were less marked in T animals; Shannon indices were lower in T 4 week-old lambs and ewes (P<0.001) and numerically lower in T younger lambs. Principal coordinates analysis were constructed independently for each age group using the weighted UniFrac similarity matrix and differentiation between T and C animals increased as age increased. These results indicate that the treatment changed the development of a conventional archaeal rumen community in young lambs without affecting VFA concentration in rumen.
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