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Biotransformation of benzo(a)pyrene in bench scale reactor using laccase of Pycnoporus cinnabarinus

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Rama, Rachel | Mougin, Christian | Boyer, François-Didier | Kollmann, Albert | Malosse, Christian | Sigoillot, Jean-Claude

Edité par CCSD ; Springer Verlag -

International audience. The biotransformation of benzo[a]pyrene by purified extracellular laccase of Pycnoporus cinnabarinus was investigated in bench scale reactors. The reaction required the presence of exogenous mediator ABTS. Most of 95% of the substrate was converted within 24 hours. The enzyme preparation oxidised the substrate mainly to benzo[a]pyrene 1,6-3,6-and 6,12-quinones in a 2/1/1 ratio after 24 hours incubations.

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