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Rotavirus-specific B cells induced by recent infection in adults and children predominantly express the intestinal homing receptor alpha 4 beta 7
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Referred to by: Corrigendum to “Rotavirus-specific B cells induced by recent infection in adults and children predominantly express the intestinal homing receptor α4β7” [Virology 305 (2003) 93–105] Virology, Volume 322, Issue 2, 1 May 2004, Page 382, Ana Marı́a Gonzalez, Marı́a C Jaimes, Isabela Cajiao, Olga L Rojas, Jean Cohen, Pierre Pothier, Evelyne Kohli, Eugene C Butcher, Harry B Greenberg, Juana Angel, Manuel A Franco. International audience. In vivo replication of rotaviruses is generally limited to enterocytes. Because of this restriction, most blood circulating rotavirus-specific B cells are hypothesized to originate in Peyer's patches and should express the intestinal homing receptor alpha4beta7. To test this hypothesis in humans, we used a flow cytometry assay that identifies antigen-activated (IgD-) B cells (CD19(+)) that express surface rotavirus-specific immunoglobulin. With this assay we could detect rotavirus-specific B cells in both children and adults with an acute rotavirus (RV) infection. Staining with an anti-alpha4beta7 monoclonal antibody, we could determine that B cells that express rotavirus-specific surface immunoglobulin predominantly express alpha4beta7. The response of rotavirus-specific antibody-secreting cells in the peripheral blood of children and adults with acute rotavirus infection was also studied by ELISPOT. The antibody-secreting cells of children were mainly of the IgM isotype, while the antibody-secreting cells of adults were predominantly of the IgA and IgG isotype. alpha4beta7+ and alpha4beta7- subsets of peripheral blood mononuclear cells were purified using paramagnetic beads and then tested in the ELISPOT assay. Rotavirus-specific antibody-secreting cells were predominantly present in the alpha4beta7+ subpopulation. The flow cytometry assay we have described will permit future studies to characterize the phenotype of virus-specific B cells and could be useful in the study of the immunogenicity and protective efficacy of RV vaccines and the identification of markers of protective immunity.
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