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Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues

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Assenberg, René | Ren, Jingshan | Verma, Anil | Walter, Thomas S. | Alderton, David | Hurrelbrink, Robert J. | Fuller, Stephen D. | Bressanelli, Stéphane | Owens, Raymond J. | Stuart, David I. | Grimes, Jonathan M.

Edité par CCSD ; Microbiology Society -

International audience. We have determined the high resolution crystal structure of the m ethylt ransfe rase domain of the NS5 polypeptide from the Murray Valley encephalitis virus. This domain is unusual in having both the N7 and 2'-O methyltransferase activity required for Cap 1 synthesis. We have also determined structures for complexes of this domain with nucleoticles and cap analogues providing information on cap binding, based on which we suggest a model of how the sequential methylation of the N7 and 2'-O groups of the cap may be coordinated.

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