Use of translational genomics to identify genes important for legume seed development

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Gallardo, Karine | d'Erfurth, Isabelle | Le Signor, Christine | Vernoud, Vanessa | Aubert, Gregoire, G. | Buitink, Julia | Gouzy, Jerome | Prosperie, Jean-Marie | Burstin, Judith | Thompson, Richard

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BAP Pôle GEAPSI. International audience. We have exploited the extensive synteny between the model legume Medicago truncatula and the cultivated garden pea, Pisumsativum, to identify loci controlling seed filling and seedcomposition in the crop species. QTLs for these traits are mapped by analyzing variation with in collections of recombinant inbred lines. Candidate genes with in the QTL intervals are identified by reference to the M.truncatula genomic sequence. This approach was used to uncover an endosperm subtilase that is associated with syntenic seed weight QTLs in Medicago and pea, and we discuss the possible role played by this enzyme in contributing to final seed weight.

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