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Covalently linked hopanoid-lipid A improves outer-membrane resistance of a Bradyrhizobium symbiont of legumes

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Silipo, Alba | Vitiello, Giuseppe | Gully, Djamel | Sturiale, Luisa | Chaintreuil, Clémence | Fardoux, Joel | Gargani, Daniel | Lee, Hae-In | Kulkarni, Gargi | Busset, Nicolas | Marchetti, Roberta | Palmigiano, Angelo | Moll, Herman | Engel, Regina | Lanzetta, Rosa | Paduano, Luigi | Parrilli, Michelangelo | Chang, Woo-Suk | Holst, Otto | Newman, Dianne K. | Garozzo, Domenico | d'Errico, Gerardino | Giraud, Eric | Molinaro, Antonio

Edité par CCSD ; Nature Publishing Group -

International audience. Lipopolysaccharides (LPSs) are major components of the outer membrane of Gram-negative bacteria and are essential for their growth and survival. They act as a structural barrier and play an important role in the interaction with eukaryotic hosts. Here we demonstrate that a photosynthetic Bradyrhizobium strain, symbiont of Aeschynomene legumes, synthesizes a unique LPS bearing a hopanoid covalently attached to lipid A. Biophysical analyses of reconstituted liposomes indicate that this hopanoid-lipid A structure reinforces the stability and rigidity of the outer membrane. In addition, the bacterium produces other hopanoid molecules not linked to LPS. A hopanoid-deficient strain, lacking a squalene hopene cyclase, displays increased sensitivity to stressful conditions and reduced ability to survive intra-cellularly in the host plant. This unusual combination of hopanoid and LPS molecules may represent an adaptation to optimize bacterial survival in both free-living and symbiotic states.

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