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Mirror slides for high-sensitivity cell and tissue fluorescence imaging

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Le Moal, Eric | Fort, Emmanuel | Lévêque-Fort, Sandrine | Janin, Anne | Murata, Hideyuki | Cordelières, Fabrice | Fontaine-Aupart, Marie-Pierre | Ricolleau, Christian

Edité par CCSD ; Society of Photo-optical Instrumentation Engineers -

International audience. Fluorescence microscopy has become the method of choice in the majority of life-science applications. We describe development and use of mirror slides to significantly enhance the fluorescence signal using standard air microscope objectives. This technique offers sufficient gain to achieve high-sensitivity imaging, together with wide field of observation and large depth of focus, two major breakthroughs for routine analysis and high-throughput screening applications on cells and tissue samples.

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