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Freezing and storage at −20 °C provides adequate preservation of Toxoplasma gondii DNA for retrospective molecular analysis

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Delhaes, Laurence | Filisetti, Denis | Brenier-Pinchart, Marie-Pierre | Pelloux, Hervé | Yera, Hélène | Dalle, Frédéric | Sterkers, Yvon | Varlet-Marie, Emmanuelle | Touafek, Feriel | Cassaing, Sophie | Bastien, Patrick

Edité par CCSD ; Elsevier -

Equipe EA MERS. International audience. Nucleic acid-based testing has become crucial for toxoplasmosis diagnosis. For retrospective (forensic or scientific) studies, optimal methods must be employed for DNA long-term storage. We compared Toxoplasma gondii detection before and after DNA storage using real-time PCR. No significant differences were found depending on duration or storage conditions at -20 °C or -80 °C.

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