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Investigation of urban birds as source of β-lactamase-producing Gram-negative bacteria in Marseille city, France
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Edité par CCSD ; Danske Dyrlaegeforening -
International audience. Background: We investigate here the presence of multidrug‑resistant bacteria isolated from stool samples of yellow‑legged gulls and chickens (n = 136) in urban parks and beaches of Marseille, France. Bacterial isolation was performedon selective media, including MacConkey agar with ceftriaxone and LBJMR medium. Antibiotic resistance genes,including extended‑spectrum β‑lactamases (ESBL) (i.e. blaCTX‑M, blaTEM and blaSHV), carbapenemases (blaKPC, blaVIM,blaNDM, blaOXA‑23, blaOXA‑24, blaOXA‑48 and blaOXA‑58) and colistin resistance genes (mcr‑1 to mcr‑5) were screened by real‑time PCR and standard PCR and sequenced when found.Results: Of the 136 stools samples collected, seven ESBL‑producing Gram‑negative bacteria (BGN) and 12 colistin‑resistant Enterobacteriaceae were isolated. Among them, five ESBL‑producing Escherichia coli and eight colistin‑resistant Hafnia alvei strains were identified. Four blaTEM‑1 genes were detected in yellow‑legged gulls and chickens.Three CTX‑M‑15 genes were detected in yellow‑legged gulls and pigeons, and one CTX‑M‑1 in a yellow‑legged gull.No mcr‑1 to mcr‑5 gene were detected in colistin‑resistant isolates. Genotyping of E. coli strains revealed four differentsequence types already described in humans and animals and one new sequence type.Conclusions: Urban birds, which are believed to have no contact with antibiotics appear as potential source of ESBLgenes. Our findings highlight the important role of urban birds in the proliferation of multidrug‑resistant bacteria andalso the possible zoonotic transmission of such bacteria from wild birds to humans.
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