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A dimerization-based fluorogenic dye-aptamer module for RNA imaging in live cells

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Bouhedda, Farah | Fam, Kyong Tkhe | Collot, Mayeul | Autour, Alexis | Marzi, Stefano | Klymchenko, Andrey | Ryckelynck, Michael

Edité par CCSD ; Nature Publishing Group -

International audience. 17 Live-cell imaging of RNA has remained a challenge because of the lack of naturally fluorescent RNAs. 18 Recently developed RNA aptamers that can light-up small fluorogenic dyes could overcome this 19 limitation, but they still suffer from poor brightness and photostability. Here, we propose a concept of 20 cell-permeable fluorogenic dimer of sulforhodamine B dyes (Gemini-561) and corresponding dimerized 21 aptamer (o-Coral) that can drastically enhance performance of the current RNA imaging method. The 22 unprecedented brightness and photostability together with high affinity of this complex allowed, for the 23 first time, direct fluorescence imaging in live mammalian cells of RNA polymerase-III transcription 24

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