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Crystallization and preliminary X-ray crystallographic analysis of Human Geminin Coiled-coil domain

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Thépaut, Michael | Dumas, Christian | Calas, Bernard | Strub, Marie-Paule | Padilla, André

Edité par CCSD ; Elsevier -

International audience. Initially discovered in Xenopus laevis, Geminin is a DNA replication initiation inhibitor found in higher eukaryotes. The coiled-coil domain of Human Geminin (termed GemH-37) has been crystallized by the vapor-diffusion sitting-drop method. A complete 1.74 Å data set has been collected on a single orthorhombic crystal with unit cell parameters a = 25.25, b = 44.35, c = 68.58 Å. Successful molecular replacement shows that GemH-37 is a dimeric parallel coiled-coil. Structural analysis is now in progress. D

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