Antiretroviral-naive and -treated HIV-1 patients can harbour more resistant viruses in CSF than in plasma
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Soulié, Cathia | Descamps, Diane | Ronique Schneider, Vé | Trabaud, Mary-Anne | Morand-Joubert, Laurence | Delaugerre, Constance | Montes, Brigitte | Barin, Francis | Ferré, Virginie | Maillard, Anne | Vallet, Sophie | Roussel, Catherine | Assoumou, Lambert | Calvez, Vincent | Flandre, Philippe | Ve, Anne-Geneviè | Jeulin, H. | Guigon, A. | Lagier, E. | Le Guillou, H. | Alloui, C. | Bettinger, D. | Pallier, C. | Fleury, H. | Reigadas, S. | Bellecave, P. | Recordon-Pinson, P. | Payan, C. | Vabret, A. | Henquell, C. | Mirand, A. | Bouvier-Alias, M. | de Rougemont, Alexis | dos Santos, G. | Morand, P. | Signori-Schmuck, A. | Bocket, L. | Rogez, S. | Andre, P. | Tardy, J. | Tamalet, C. | Delamare, C. | Schvoerer, E. | André-Garnier, E. | Cottalorda, J. | Guinard, J. | Guiguon, A. | Brun-Vezinet, F. | Charpentier, C. | Visseaux, B. | Peytavin, G. | Krivine, A. | Si-Mohamed, A. | Avettand-Fenoel, V. | Marcelin, A.-G. | Lambert-Niclot, S. | Wirden, M. | Chaix, M. | Amiel, C. | Schneider, V. | Giraudeau, G. | Brodard, V. | Plantier, J. | Chaplain, C. | Bourlet, T. | Fafi-Kremer, S. | Stoll-Keller, F. | Schmitt, M. | Barth, H. | Yerly, S. | Poggi, C. | Izopet, Jacques | Raymond, S. | Chaillon, A. | Marque-Juillet, S. | Roque-Afonso, A. | Haim-Boukobza, S. | Grude, Maxime | Costagliola, D. | Allegre, T. | Schmit, J. | Chennebault, J. | Bouchaud, O. | Magy-Bertrand, N. | Delfraissy, J. | Dupon, M. | Morlat, P. | Neau, D. | Ansart, S. | Jaffuel, S. | Verdon, R. | Jacomet, C. | Levy, Y. | Dominguez, S. | Chavanet, P. | Piroth, L. | Cabié, A. | Leclercq, P. | Ajana, F. | Cheret, A. | Weinbreck, P. | Cotte, L. | Poizot-Martin, I. | Ravaud, I. | Christian, B. | Truchetet, F. | Grandidier, M. | Reynes, J. | May, T. | Goehringer, F. | Raffi, F. | Dellamonica, P. | Prazuck, T. | Hocqueloux, L. | Yeni, P. | Landman, R. | Launay, O. | Weiss, L. | Viard, J. | Katlama, C. | Simon, A. | Girard, Pascal | Meynard, J. | Molina, J. | Pialoux, G. | Hoen, B. | Goeger-Sow, M. | Lamaury, I. | Beaucaire, G. | Jaussaud, R. | Rouger, C. | Michelet, C. | Borsa-Lebas, F. | Caron, F. | Khuong, M. | Lucht, F. | Rey, D. | Calmy, A. | Marchou, B. | Gras, G. | Greder-Belan, A. | Vittecoq, D. | Teicher, E.
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CCSD ; Oxford University Press (OUP) -
International audience.
Objectives: The neurological disorders in HIV-1-infected patients remain prevalent. The HIV-1 resistance in plasma and CSF was compared in patients with neurological disorders in a multicentre study. Methods: Blood and CSF samples were collected at time of neurological disorders for 244 patients. The viral loads were .50 copies/mL in both compartments and bulk genotypic tests were realized. Results: On 244 patients, 89 and 155 were antiretroviral (ARV) naive and ARV treated, respectively. In ARV-naive patients, detection of mutations in CSF and not in plasma were reported for the reverse transcriptase (RT) gene in 2/89 patients (2.2%) and for the protease gene in 1/89 patients (1.1%). In ARV-treated patients, 19/152 (12.5%) patients had HIV-1 mutations only in the CSF for the RT gene and 30/151 (19.8%) for the protease gene. Two mutations appeared statistically more prevalent in the CSF than in plasma: M41L (P ¼ 0.0455) and T215Y (P ¼0.0455). Conclusions: In most cases, resistance mutations were present and similar in both studied compartments. However, in 3.4% of ARV-naive and 8.8% of ARV-treated patients, the virus was more resistant in CSF than in plasma. These results support the need for genotypic resistance testing when lumbar puncture is performed.
