Afficher la couverture 'Preferential insertion of lactose permease in phospholipid domains: AFM observations | Picas, Laura' en grand format
/5

0 avis

Preferential insertion of lactose permease in phospholipid domains: AFM observations

Archive ouverte

Picas, Laura | Carretero-Genevrier, Adrian | Montero, M Teresa | Vázquez-Ibar, J.L. | Seantier, Bastien | Milhiet, Pierre-Emmanuel | Hernández-Borrell, Jordi | Montero, M. Teresa

Edité par CCSD ; Elsevier -

International audience. We report the insertion of a transmembrane protein, lactose permease (LacY) from Escherichia coli (E. coli), in supported lipid bilayers (SLBs) of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG), in biomimetic molar proportions. We provide evidence of the preferential insertion of LacY in the fluid domains. Analysis of the self-assembled protein arrangements showed that LacY: (i) is inserted as a monomer within fluid domains of SLBs of POPE:POPG (3:1, mol/mol), (ii) has a diameter of approx. 7.8nm; and (iii) keeps an area of phospholipids surrounding the protein that is compatible with shells of phospholipids.

Consulter en ligne

Suggestions

Du même auteur

Calcium-Induced Formation of Subdomains in Phosphatidylethanolamine−Phosphatidylglycerol Bilayers: A Combined DSC, 31 P NMR, and AFM Study

Archive ouverte | Picas, Laura | CCSD

International audience. We study the effect of Ca(2+) on the lateral segregation of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG) (3:1, mol...

Evidence of phosphatidylethanolamine and phosphatidylglycerol presence at the annular region of lactose permease of Escherichia coli

Archive ouverte | Picas, Laura | CCSD

International audience. Biochemical and structural work has revealed the importance of phospholipids in biogenesis, folding and functional modulation of membrane proteins. Therefore, the nature of protein-phospholip...

Atomic force microscopy characterization of supported planar bilayers that mimic the mitochondrial inner membrane

Archive ouverte | Domènech, Oscar | CCSD

International audience. In this study we examined the properties of supported planar bilayers (SPBs) formed from phospholipid components that comprise the mitochondrial inner membrane. We used 1-palmitoyl-2-oleoyl-s...

Chargement des enrichissements...