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Cloning and expression of a single-chain antibody fragment specific for a monomorphic determinant of class I molecules of the human major histocompatibility complex

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Marin, M. | Brockly, F. | Noel, D. | Etienne-Julan, M. | Biard-Piechaczyk, M. | Hua, T. D. | Gu, Z. J. | Piechaczyk, Marc

Edité par CCSD ; Mary Ann Liebert -

International audience. B9.12.1 is a monoclonal antibody specific for a monomorphic determinant of human MHC class I molecules. It is currently used for cell typing and is useful for targeting infection of human cells by murine ecotropic retroviruses. We have cloned and expressed it in the form of a single-chain variable fragment (ScFv) that recognizes the same epitope as the parental antibody. Through genetic engineering, this ScFv may be used for developing new cell-typing probes and new retroviral targeting approaches.

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