A split fluorescent reporter with rapid and reversible complementation

Archive ouverte

Tebo, Alison, G | Gautier, Arnaud

Edité par CCSD ; Nature Publishing Group -

International audience. Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorogenic reporter FAST (Fluorescence-Activating and absorption-Shifting Tag), which specifically and reversibly binds fluorogenic hydroxybenzylidene rhodanine (HBR) analogs, splitFAST displays rapid and reversible com-plementation, allowing the real-time visualization of both the formation and the dissociation of a protein assembly.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion