Protein crystal structure determination with the crystallophore, a nucleating and phasing agent

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Engilberge, Sylvain | Wagner, Tristan | Santoni, Gianluca | Breyton, Cécile | Shima, Seigo | Franzetti, Bruno | Riobé, François | Maury, Olivier | Girard, Eric

Edité par CCSD ; International Union of Crystallography -

International audience. Obtaining crystals and solving the phase problem remain major hurdles encountered by bio-crystallographers in their race to obtain new high-quality structures. Both issues can be overcome by the crystallophore, Tb-Xo4, a lanthanide-based molecular complex with unique nucleating and phasing properties. This article presents examples of new crystallization conditions induced by the presence of Tb-Xo4. These new crystalline forms bypass crystal defects often encountered by crystallographers, such as low-resolution diffracting samples or crystals with twinning. Thanks to Tb-Xo4's high phasing power, the structure determination process is greatly facilitated and can be extended to serial crystallography approaches

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