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HIV-1 Antisense Transcription Is Preferentially Activated in Primary Monocyte-Derived Cells

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Laverdure, Sylvain | Gross, Antoine | Arpin-André, Charlotte | Clerc, Isabelle | Beaumelle, Bruno | Barbeau, Benoit | Mesnard, Jean-Michel

Edité par CCSD ; American Society for Microbiology -

International audience. In this study, an antisense luciferase-expressing human immunodeficiency virus type 1 (HIV-1) molecular clone was used to infect primary cells. We found that antisense transcription activity from the 3' long terminal repeat (LTR) was significantly more abundant in monocyte-derived cells than in activated T lymphocytes. Moreover, by analyzing antisense transcription in infected monocyte-derived dendritic cells (MDDCs), we observed that the majority of HIV-1-infected MDDCs with significant antisense transcription activity did not produce Gag. We also confirmed that the negative-strand-encoded antisense protein (ASP) was expressed in monocyte-derived cells.

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