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Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics

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Bancelin, Stéphane | Lynch, Barbara | Bonod-Bidaud, Christelle | Dokládal, Petr | Ruggiero, Florence | Allain, Jean-Marc | Schanne-Klein, Marie-Claire

Edité par CCSD ; Humana Press -

International audience. An important issue in tissue biomechanics is to decipher the relationship between the mechanical behavior at macroscopic scale and the organization of the collagen fiber network at microscopic scale. Here, we present a protocol to combine traction assays with multiphoton microscopy in ex vivo murine skin. This multiscale approach provides simultaneously the stress/stretch response of a skin biopsy and the collagen reorganization in the dermis by use of second harmonic generation (SHG) signals and appropriate image processing.

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