Afficher la couverture 'Automating multimodal microscopy with NanoJ-Fluidics | Almada, Pedro' en grand format
/5

0 avis

Automating multimodal microscopy with NanoJ-Fluidics

Archive ouverte

Almada, Pedro | Pereira, Pedro M. | Culley, Siân | Caillol, Ghislaine | Boroni-Rueda, Fanny | Dix, Christina, L | Baum, Buzz | Laine, Romain, F | Leterrier, Christophe | Henriques, Ricardo

Edité par CCSD ; Nature Publishing Group -

International audience. Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolved live-to-fixed and multiplexed STORM/DNA-PAINT experiments.

Suggestions

Du même auteur

NanoJ: a high-performance open-source super-resolution microscopy toolbox

Archive ouverte | Laine, Romain, F | CCSD

International audience. Super-resolution microscopy (SRM) has become essential for the study of nanoscale biological processes. This type of imaging often requires the use of specialised image analysis tools to proc...

High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation

Archive ouverte | Laine, Romain, F | CCSD

High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation

Archive ouverte | Laine, Romain, F | CCSD

International audience. Live-cell super-resolution microscopy enables the imaging of biological structure dynamics below the diffraction limit. Here we present enhanced super-resolution radial fluctuations (eSRRF), ...

Chargement des enrichissements...