Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies

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Nurtop, Elif | Villarroel, Paola Mariela Saba | Pastorino, Boris | Ninove, Laetitia | Drexler, Jan Felix | Roca, Yelin | Gake, Bouba | Dubot-Pérès, Audrey | Grard, Gilda | Peyrefitte, Christophe, N | Priet, Stephane | de Lamballerie, Xavier | Gallian, Pierre

Edité par CCSD ; BioMed Central -

International audience. Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies.

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