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Time-resolved measurement of the electric field induced by a plasma gun device in a conventional electroporation setup
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International audience. An organic tissue exposed to plasma experiences a highly reactive environment consisting of ions,electrons, free radicals, UV radiation, neutral molecules and electric field (EF). Of these activeagents, EF is one of the least investigated up to these days [1]. Nevertheless, EF can potentiallyplay a major role transiently increasing cell membrane permeability, a phenomenon commonlyknown as “electroporation” [2] and nowadays effectively used for several applications includinggene transfection and electrochemotherapy [3]. Thus, in the present work, we report the timeresolved measurement of the EF induced in a liquid medium within a typical electroporation setupby a plasma gun (PG) device. The results are compared with the values estimated for aconventional electroporation device (Cliniporator, IGEA SpA) in the perspective of a possibleimpact of plasma induced EF on biological targets. The comparison was done adopting a 4 wellμ-slide (Ibidi®) armed with two plane parallel electrodes used for in-vitro electroporation test andfilled with 500μl of the target liquid, being either SMEM culture medium or high purity water.Three different experimental cases were taken into account (Fig.1, left): 1) the plane electrodespowered by the Cliniporator; 2) the PG plume hitting on one plane electrode; 3) the PG plumehitting on the liquid with (3a) and without (3b) ground electrode. Despite the considerably highervoltage powering the plasma device, preliminary results show an EF on the target lower than theone applied by the electroporation system. This observation goes along with the recorded currentthat results in general one order of magnitude lower for the plasma system. Furthermore, resultsshow a key role played by the grounding of the liquid medium which can hinder the induction ofan EF by the plasma source. While still preliminary, presented results provide new insights on oneof the less investigated aspects of plasma and liquid target interaction, the induced EF.Fig. 1: (left) Photos and schematics of the experimental set up; voltage, current and EF waveformfor case 1 (center) and case 3b (right).This work and A.S. are supported by PLASCANCER project, INCa-PlanCancer-n°17CP087-00References[1] E. Robert, T. Darny, S. Dozias, S. Iseni, J. M. Pouvesle, Physics of Plasma, 22, 122007 (2015).[2] D. Miklavćić; M. Puc, Wiley Encyclopedia of Biomedical Engineering, 1 (2006).[3] L. M. Mir, European J. of Cancer Supplement, 4, 38-44 (2006)
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