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Projection map of cytochrome b6 f complex at 8 A resolution.

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Mosser, Gervaise | Breyton, Cécile | Olofsson, Anders | Popot, Jean-Luc | Rigaud, Jean-Louis

Edité par CCSD ; American Society for Biochemistry and Molecular Biology -

International audience. The structure of the cytochrome b6 f complex has been investigated by electron microscopy and image analysis of thin three-dimensional crystals. Electron micrographs of negatively stained specimens were recorded and showed optical diffraction peaks to 10 A resolution. A projection map was calculated at 8 A resolution and showed the presence of cytochrome b6 f dimers. The extramembrane part of each monomer featured a C shape, with mean external diameter approximately of 53 A and an internal groove approximately 14 A long and approximately 9 A wide. Within each monomer, strong features were clearly resolved and tentatively attributed to some of the subunits of the cytochrome b6 f complex. The data are consistent with the Rieske iron-sulfur protein lying close to the monomer-monomer interface and the heme-bearing domain of cytochrome f far from it.

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