Ensuring the safety and security of frozen lung cancer tissue collections through the encapsulation of dried DNA

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Washetine, K. | Kara-Borni, M. | Heeke, S. | Bonnetaud, C. | Félix, J.-M. | Ribeyre, L. | Bence, C. | Ilie, M. | Bordone, O. | Pedro, M. | Maitre, P. | Tanga, V. | Gormally, E. | Mossuz, P. | Lorimier, Philippe | Marquette, C.H. | Mouroux, J. | Cohen, C. | Lassalle, S. | Long-Mira, E. | Clément, B. | Dagher, G. | Hofman, Véronique | Hofman, Paul

Edité par CCSD ; MDPI -

International audience. Collected specimens for research purposes may or may not be made available depending on their scarcity and/or on the project needs. Their protection against degradation or in the event of an incident is pivotal. Duplication and storage on a different site is the best way to assure their sustainability. The conservation of samples at room temperature (RT) by duplication can facilitate their protection. We describe a security system for the collection of non-small cell lung cancers (NSCLC) stored in the biobank of the Nice Hospital Center, France, by duplication and conservation of lyophilized (dried), encapsulated DNA kept at RT. Therefore, three frozen tissue collections from non-smoking, early stage and sarcomatoid carcinoma NSCLC patients were selected for this study. DNA was extracted, lyophilized and encapsulated at RT under anoxic conditions using the DNAshell technology. In total, 1974 samples from 987 patients were encapsulated. Six and two capsules from each sample were stored in the biobanks of the Nice and Grenoble (France) Hospitals, respectively. In conclusion, DNA maintained at RT allows for the conservation, duplication and durability of collections of interest stored in biobanks. This is a low-cost and safe technology that requires a limited amount of space and has a low environmental impact. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.

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