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Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser

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Ekeberg, Tomas | Kauffmann, Martin | Abergel, Chantal | Maia, Filipe | Seltzer, Virginie | Claverie, Jean-Michel | Hantke, Max | Jönsson, Olof | Nettelblad, Carl | van Der Schot, Gijs | Liang, Mengning | Deponte, Daniel | Barty, Anton | Seibert, M Marvin | Iwan, Bianca | Andersson, Inger | Loh, N Duane | Martin, Andrew | Chapman, Henry | Bostedt, Christoph | Bozek, John | Ferguson, Ken | Krzywinski, Jacek | Epp, Sascha | Rolles, Daniel | Rudenko, Artem | Hartmann, Robert | Kimmel, Nils | Hajdu, Janos | Maia, Filipe R. n. c. | Seibert, M. Marvin | Loh, N. Duane

Edité par CCSD ; American Physical Society -

International audience. We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.

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