Syracuse

Online Raman spectroscopy for structural biology on beamline ID29 of the ESRF

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von Stetten, David | Giraud, Thierry | Bui, Soi | Steiner, Roberto | Fihman, François | de Sanctis, Daniele | Royant, Antoine

Edité par CCSD ; Elsevier -

International audience. Raman spectroscopy can probe the structure and conformations of specific chemical groups within proteins and may thus be used as a technique complementary to X-ray crystallography. This combined approach can be decisive in resolving ambiguities in the interpretation of enzymatic or X-ray induced processes. Here, we present an online Raman setup developed at the European Synchrotron that allows for interleaved Raman spectra acquisition and X-ray diffraction measurements with fast probe exchange and simple alignment while maintaining a high sensitivity over the entire spectral range. This device has been recently employed in the study of a covalent intermediate in the O2-dependent breakdown of uric acid by the cofactor-free enzyme urate oxidase and to monitor its decay induced by X-ray exposure.

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