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Deciphering Cell Intrinsic Properties: A Key Issue for Robust Organoid Production

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Picollet-d'Hahan, Nathalie | Dolega, Monika E | Freida, Delphine | Martin, Donald K. | Gidrol, Xavier, E

Edité par CCSD ; Elsevier -

International audience. We highlight the disposition of various cell types to self-organize into complexorgan-like structures without necessarily the support of any stromal cells,provided they are placed into permissive 3D culture conditions. The goal ofgenerating organoids reproducibly and efficiently has been hampered by poorunderstanding of the exact nature of the intrinsic cell properties at the origin oforganoid generation, and of the signaling pathways governing their differen-tiation. Using microtechnologies like microfluidics to engineer organoidswould create opportunities for single-cell genomics and high-throughputfunctional genomics to exhaustively characterize cell intrinsic properties. Amore complete understanding of the development of organoids wouldenhance their relevance as models to study organ morphology, function,and disease and would open new avenues in drug development and regener-ative medicine.

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